2024年

原著​​​​

• Ebina, M., Miura, Y. and Sakane, F. Ubiquitin-specific peptidase 11 selectively interacts with and deubiquitination-dependently stabilizes diacylglycerol kinase δ to maintain cellular glucose uptake. Biochim. Biophys. Acta - Mol. Cell Res., in press

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• Sakai, H., Murakami, C., Takechi, M., Urano, T. and Sakane, F. Diacylglycerol kinase δ is required for skeletal muscle development and regeneration. FASEB BioAdvances, in press

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• Murakami, C., Dilimulati, K., Atsuta‐Tsunoda, K., Kawai, T., Inomata, S., Hijikata, Y., Sakai, H., and Sakane F. Multiple activities of sphingomyelin synthase 2 generate saturated fatty acid- and/or monounsaturated fatty acid-containing diacylglycerol. Journal of Biological Chemistry (in  press)
  DOI: 10.1016/j.jbc.2024.107960​　　プレスリリース

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総説

• Sakane F., Murakami, C. and Sakai, H.: Upstream and Downstream Pathways of Diacylglycerol Kinase Novel Phosphatidylinositol Turnover-independent Signal Transduction Pathways. Advances in Biological Regulation, 101054 (in press)
  DOI: https://doi.org/10.1016/j.jbior.2024.101054

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2023年

原著​​​​

• Suzuki, R., Murakami, C., Dilimulati, K., Atsuta‐Tsunoda, K., Kawai, T. and Sakane F. Human sphingomyelin synthase 1 generates diacylglycerol in the presence and absence of ceramide via multiple enzymatic activities.  FEBS Letters, 597 (21) 2672–2686 (2023)
  DOI: https://doi.org/10.1002/1873-3468.14735

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• Furuta, M., Murakami, C., Numagami, Y., Suzuki, R. and Sakane, F. Diacylglycerol kinase ζ interacts with sphingomyelin synthase 1 and sphingomyelin synthase-related protein via different regions. FEBS Open Bio, 13 (7), 1333–1345 (2023) (DOI: 10.1002/2211-5463.13628)

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• Yamazaki, A., Kawashima, A., Honda, T., Kohhama, T., Murakami, C., Sakane, F., Murayama, T. and Nakamura, H. Identification and characterization of diacylglycerol kinase ζ as a novel enzyme producing ceramide-1-phosphate. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1868 (6), 159307 (2023) (DOI: 10.1016/j.bbalip.2023.159307)

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• Yachida, N., Hoshino, F., Murakami, C., Ebina, M., Miura, Y. and Sakane, F. Saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids selectively interacts with heat shock protein 27. J. Biol. Chem., 299 (3), Article 103019 (2023) (DOI: 10.1016/j.jbc.2023.103019)

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• Numagami, Y., Hoshino, F., Murakami, C., Ebina, M. and Sakane, F. Distinct regions of Praja-1 E3 ubiquitin-protein ligase selectively bind to docosahexaenoic acid-containing phosphatidic acid and diacylglycerol kinase δ. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1868 (3)159265 (2023) (DOI: 10.1016/j.bbalip.2022.159265)

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2022年

原著​​​​​

• Hoshino, F., Nakayama, M., Furuta, M., Murakami, C., Kato, A. and *Sakane, F. Phosphatidylinositol 4,5-bisphosphate-specific phospholipaseC β1 selectively binds dipalmitoyl dipalmitoyl and distearoylphosphatidic acids via Lys946 and Lys951. Lipids, 57 (6), 289–302 (2022) (DOI: 10.1002/lipd.12356)

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• Sakai, H., Matsumoto, K., Urano, T. and Sakane, F. Myristic acidselectively augments β-tubulin levels in C2C12 myotubes via diacylglycerol kinase δ. FEBS Open Bio, 12 (10), 1788–1796 (2022) (DOI: 10.1002/2211-5463.13466)

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• Takahashi, D., Yonezawa, K., Okizaki, Y., Shimada, M., Caarveiro J. M. M., Ueda, T., Shimada, A., Sakane, F. and Shimizu, N. Ca2+-inducedstructural changes and intramolecular interactions in N-terminal regionof diacylglycerol kinase alpha. Protein Sci., 31 (7), e4365 (2022) (DOI: 10.1002/pro.4365)

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• Okada, N., Sugiyama, K., Shichi, S., Shirai, Y., Goto, K., Sakane, F., Kitamura, H. and Taketomi, A. Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade. Cancer Immunol. Immunother., 71 (4), 889–903 (2022) (DOI: 10.1007/s00262-021-03041-z)

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• Masuda, Y., Murakami, C., Suzuki, R. and Sakane, F. Diacylglycerol kinase η regulates cell proliferation and its levels are elevated by glucocorticoids in undifferentiated neuroblastoma cells. Biochem. Biophys. Res. Commun., 602, 41–48 (2022) (DOI: 10.1016/j.bbrc.2022.02.100)

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• Hoshino, F. and Sakane, F. Docosahexaenoic acid-containing phosphatidic acid interacts with clathrin coat assembly protein AP180 and regulates its interaction with clathrin. Biochem. Biophys. Res. Commun., 587 (1), 69–77 (2022) (DOI: 10.1016/j.bbrc.2021.11.097)

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2021年

原著​​

• Nakano, T., Tanaka, T., Sakane, F., Kaneko, M. K., Kato, Y. and Goto, K. Immunocytochemical Analysis of DGKη in Cultured Cells using a Monoclonal Antibody DhMab-4. Monoclon. Antib. Immunodiagn. Immunother., 40 (6), 261–265 (2021) (DOI: 10.1089/mab.2021.0034)

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• Hoshino, F. and Sakane, F. The SAC1 phosphatase domain of synaptojanin-1 is activated by interacting with polyunsaturated fatty acid-containing phosphatidic acids. FEBS Lett., 595 (19), 2479-2492 (2021) (DOI: 10.1002/1873-3468.14177)

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• Bozelli, J. C., Yone, J., Takahashi, D. Sakane, F. and Epand R. M. Membrane morphology determines diacylglycerol kinase α substrate acyl chain specificity. FASEB J., 35 (6), e21602 (2021) (DOI: 10.1096/fj. 202100264R)

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• Asami, M., Suzuki, Y. and Sakane, F. Dopamine and phosphorylated dopamine transporter increase in the diacylglycerol kinase η-knockout mouse brain. FEBS Lett., 595 (9), 1313–1321 (2021) (DOI: 10.1002/1873-3468.14059)​, Selected as a highlight to feature on the slider (highlights showcase articles)

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• Suzuki, Y., Asami, M., Takahashi, D. and Sakane, F. Diacylglycerol kinase η colocalizes and interacts with apoptosis signal-regulating kinase 3 in response to osmotic shock. Biochem. Biophys. Rep., 26, 101006 (2021) (DOI:10.1016/j.bbrep.2021.101006)​

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• Murakami, C. and Sakane, F. Sphingomyelin synthase-related protein generates diacylglycerol via the hydrolysis of glycerophospholipids in the absence of ceramide. J. Biol. Chem., 296, 100454 (2021) (DOI: 10.1016/j.jbc.2021.100454)

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• Ishizaki, A., Murakami, C., Yamada, H. and Sakane, F. Diacylglycerol kinase η activity in cells using protein myristoylation and cellular phosphatidic acid-sensor. Lipids, 56 (4), 449–458 (2021) (DOI: 10.1002/lipd.12301)

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• Sato, T., Ishiwatari, C., Kaneko, Y. K., Nakayama, T., Ishikawa, Y., Kimura, Y., Matsuda, Y., Chiba, R., Fujinuki, T., Iwata, K., Lu, Q., Usuki, T., Sakane, F., and Ishikawa, T. Diacylglycerol kinase δ functions as a proliferation suppressor in pancreatic β-cells. FASEB J., 35 (5), e21420 (2021) (DOI: 10.1096/fj.202001279RR)

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• Takao, S., Akiyama, R. and Sakane, F. Combined inhibition/silencing of diacylglycerol kinase α and ζ simultaneously and synergistically enhances interleukin-2 production in T cells and induces cell death of melanoma cells. J. Cell. Biochem., 122 (5), 494–506 (2021)　

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総説

• Sakane, F., Hoshino, F., Ebina, M., Sakai, H. and Takahashi, D. The roles of diacylglycerol kinase α in cancer cell proliferation and apoptosis. Special Issue "Diacylglycerol Kinases in Cancer”, Cancers, 13,  5190 (2021)

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• 坂根郁夫，村上千明，星野史規，多彩な生理病理現象を制御する新奇ジアシルグリセロールリン酸化経路群．月刊「アグリバイオ」， Vol. 5 (2), 71－77 (173－179) (2021)Link

 

2020年

原著　　　　　　　　　　　　　　　　　

• Sakai, H., Murakami, C., Usuki, T., Lu, Q., Matsumoto, K., Urano, T. and Sakane, F. Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway. Biochimie, 177, 13–24 (2020)

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• Hoshino, F. and Sakane, F. Polyunsaturated fatty acid-containing phosphatidic acids selectively interact with L-lactate dehydrogenase A and induces its secondary structural change and inactivation. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1865 (10), Article 158768 (2020

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• Yamada, H. Hoshino, F., Lu, Q. and Sakane, F. Cellular phosphatidic acid sensor, α-synuclein N-terminal domain, detects endogenous phosphatidic acid in macrophagic phagosomes and neuronal growth cone. Biochem. Biophys. Rep., 22, Article 100769 (2020)

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• Murakami, C., Hoshino, F., Sakai, H., Hayashi, Y., Yamashita, A. and Sakane, F. Diacylglycerol kinase δ and sphingomyelin synthase–related protein functionally interact via their sterile α motif domains. J. Biol.
  Chem., 295 (10), 2932–2947 (2020)  *This article has been selected to appear in a special virtual issue. (Link)

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• Yamada, H., Mizuno. S., Honda, S., Takahashi, D. and Sakane, F. Characterization of α-synuclein N-terminal domain as a novel cellular phosphatidic acid sensor. FEBS J. 287 (11), 2212–2234 (2020)     *This article has been selected as Editor's Choice (Link)      

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• Lu, Q., Murakami, C., Hoshino, F., Murakami, Y. and Sakane, F. Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1865 (3), Article 158608 (2020)​   

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• Lu, Q., Murakami, C., Murakami, Y., Hoshino, F., Asami, M., Usuki, T.,Sakai, H. and Sakane, F. 1-Stearoyl-2-docosahexaenoyl-phosphatidic acid interacts with and activates Praja-1, the E3 ubiquitin ligase acting on
  the serotonin transporter in the brain. FEBS Lett., 594 (11), 1787–1796(2020)

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• Murakami, Y., Murakami, C., Hoshino, F., Lu, Q., Akiyama, R., Yamaki, A., Takahashi, D. and Sakane, F. Palmitic acid- and/or palmitoleic acid-containing phosphatidic acids are generated by diacylglycerol kinase α in starved Jurkat T cells. Biochem. Biophys. Res. Commun., 525 (4), 1054–1060 (2020)

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総説

• Sakane, F., Hoshino, F. and Murakami, C. New era of diacylglycerol kinase, phosphatidic acid and phosphatidic acid-binding protein. Special Issue “Diacylglycerol kinases in signal transduction”. Int. J. Mol. Sci. 21, Article 6794 (2020)  (Invited review) Link 　*This article has been selected as the Highly Cited Papers of IJMS in 2020.

 

2019年

原著​

• Honda, S., Murakami, C., Yamada, H., Murakami, Y., Ishizaki, A. and Sakane, F. Analytical method for diacylglycerol kinase ζ activity in cells using protein myristoylation and liquid chromatography–tandem mass spectrometry. Lipids, 54 (11–12), 763–771 (2019)

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• Komenoi. S., Suzuki, Y., Asami, M., Murakami, C., Hoshino, F., Chiba, S., Takahashi, D., Kado, S. and Sakane, F. Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. Biochem. Biophys. Rep., 19, Article 100660 (2019)

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• Hoshino, F., Murakami, C., Sakai, H., Satoh, M. and Sakane, F. Creatine kinase muscle type specifically interacts with saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids. Biochem. Biophys. Res. Commun., 513 (4), 1035–1040 (2019) ​

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• Iwata, K., Sakai, H., Takahashi, D. and Sakane F. Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1864 (7), 1031–1038 (2019)

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• Takahashi, D., Suzuki, K., Sakamoto, T., Iwamoto, T., Murata T. and Sakane, F. Crystal structure and calcium-induced conformational changes of diacylglycerol kinase α EF-hand domains. Protein Sci., 28,  694–706(2019)

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• Saito, T., Takahashi, D. and Sakane, F. Expression, purification and> characterization of human diacylglycerol kinase ζ. ACS Omega, 4, 5540–5546 (2019)

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• Hayashi, D., Tsumagari, R., Liu, K., Ueda, S., Yamanoue, M., Sakane, F. and Shirai, Y. Screening of subtype specific activators and inhibitors for diacylglycerol kinase. J. Biochem. 165 (6), 517–522 (2019)

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• Yamaki, A., Akiyama, R., Murakami, C., Takao, S., Murakami, Y., Mizuno, S., Takahashi, D., Kado, S., Taketomi, A., Shirai, Y., Goto, K. and Sakane, F. Diacylglycerol kinase α-selective inhibitors induce apoptosis and reduce viability of melanoma and several other cancer cell lines. J. Cell. Biochem., 120 (6),  10043–10056 (2019) *This article has been recognized as one of the most read in Journal of Cellular Biochemistry (Top Downloaded Paper 2018-2019).

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2018年

原著

・Nakano, T., Ogasawara, S., Tanaka, T., Hozumi, Y., Yamaki, A., Sakane, F., Shirai, Y., Nakamura, T., Yanaka, M., Yamada, S., Kaneko, M. K., Kato, Y. and Goto, K.

DgMab-6: anti-human DGKγ monoclonal antibody for immunocytochemistry. Monoclon. Antib. Immunodiagn. Immunother. 37 (5), 229–232 (2018)

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・Maeda, Y., Shibata, K., Akiyama, R., Murakami, C., Takao, S., Murakami, Y., Takahashi,  D., Sakai, H. and Sakane, F.

Diacylglycerol kinase β induces filopodium formation via its C1, catalytic and carboxy-terminal domains and interacts with the Rac1-GTPase-activating protein, β2-chimaerin. Biochem. Biophys. Res. Commun., 504 (1), 54–60 (2018)

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・Takahashi, D. and Sakane, F. Expression and purification of human diacylglycerol kinase α from baculovirus-infected insect cells for structural studies. PeerJ, 6, e5449 (2018)

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​・Sakai, H., Murakami, C., Matsumoto, K., Urano, T. and Sakane, F.

Diacylglycerol kinase δ controls down-regulation of cyclin D1 for C2C12 myogenic differentiation. Biochimie, 151, 45-53 (2018)

・Lu, Q., Komenoi, S., Usuki, T., Takahashi, D. and Sakane, F.

Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain. Biochem. Biophys. Res. Commun., 497 (4), 1031–1037 (2018)

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総説

・Sakane, F., Mizuno, S., Takahashi, D. and Sakai, H.

Where do substrates of diacylglycerol kinases come from?: Diacylglycerol kinases utilize diacylglycerol species supplied from phosphatidylinositol turnover-independent pathways. Adv. Biol. Regul., 67, 101–108 (2018)

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著書

・坂根郁夫．第６章　生物を治す：糖尿病リスクを低減する食品開発をめざす，

千葉大学ベンチャービジネスラボラトリー　斎藤恭一　編，バイオベンチャーの

冒険者たち　千葉大発！　世界をアップデートする６人のバイオ研究者，pp167－
198，幻冬舎，東京（2018）

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・坂根郁夫．牛乳に特徴的に含まれるミリスチン酸が2型糖尿病リスクを低減．一
般社団法人　Jミルク　牛乳乳製品健康科学会議　乳の学術連合「あたらしいミ
ルクの研究　2017年度」レポート（1．牛乳製品健康科学），2017年度版 pp1－4，
東京（2018）Web出版 (Link)

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2017年

原著

・Murakami, C., Mizuno, S., Kado, S. and Sakane, F. Development of a liquid chromatography-mass　spectrometry based enzyme activity assay for phosphatidylcholine-specific phospholipase C. Anal. Biochem., 526, 43–49 (2017)

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・Mizuno, S., Sasai, H., Kume, A., Takahashi, D., Satoh, M., Kado, S. and Sakane, F. Dioleoyl-phosphatidic acid selectively binds to α-synuclein and strongly induces its aggregation. FEBS Lett., 591 (5), 784–791 (2017)

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・Takato, T., Iwata, K., Murakami, C., Wada, Y. and Sakane, F. Chronic administration of myristic acid improves hyperglycaemia in the Nagoya–Shibata–Yasuda mouse model of congenital type 2 diabetes. Diabetologia, 60 (10), 2076–2083 (2017)

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・Nakano, T., Ogasawara, S., Tanaka, K., Hozumi, Y., Mizuno, S., Satoh, E., Sakane, F., Okada, N., Taketomi, A., Honma, R., Nakamura, T., Saidoh, N., Yanaka, M., Itai, S., Handa, S., Chang, Y-W., Yamada, S., Kaneko, M. K.
, Kato, Y. and Goto, K.

DaMab-2: Anti-human DGKα monoclonal antibody for immunocytochemistry. Monoclon. Antib. Immunodiagn. Immunother., 36 (4), 181–184 (2017)

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総説​

・Merida, I., Graziani, A. and Sakane, F.. Editorial: Diacylglycerol kinase. Research Topic "Diacylglycerol Kinase Signalling", Front. Cell Dev. Biol., 5, Article 84, 1–2 (2017)

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著書

・Diacylglycerol kinase signalling. ed. by Merida, I., Graziani A. and Sakane, F. Front. Cell Dev. Biol., Frontiers Media, Lausanne, Switzerland (2017), ISBN 978-2-88945-335-1

 

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2016年

原著

・Mizuno, S., Kado, S., Goto, K., Takahashi, D. and Sakane, F. Diacylglycerol kinase ζ generates dipalmitoyl-phosphatidic acid species during neuroblastoma cell differentiation. Biochem. Biophys. Rep., 8, 352–359 (2016)

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・Murakami, E., Shionoya, T. Komenoi, S., Suzuki, Y. and Sakane, F.Cloning and characterization of novel testis-specific diacylglycerol kinase η splice variant 3 and 4. PLoS One 11 (9), Article e0162997, 1–14 (2016)

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・Usuki, T., Takato, T., Lu Q., Sakai, H., Bando, K., Kiyonari, H. and Sakane, F. Behavioral and pharmacological phenotypes of brain-specific diacylglycerol kinase δ-knockout mice. Brain Res. 1648, 193–201 (2016)

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・Kamiya, Y., Mizuno, S., Komenoi, S., Sakai, H., and Sakane, F., Activation of conventional and novel protein kinase C isozymes by different diacylglycerol molecular species. Biochem. Biophys. Rep. 7, 361–366 (2016)

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・Isozaki, T., Komenoi, S., Lu Q., Usuki, T., Tomokata, S., Matsutomo, D., Sakai, H. Bando, K., Kiyonari, H. and Sakane, F. Deficiency of diacylglycerol kinase η induces lithium-sensitive mania-like behaviors. J. Neurochem. 138 (3), 448–456 (2016)

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・Wada, Y., Sakiyama, S., Sakai, H. and Sakane, F. Myristic acid enhances diacylglycerol kinase δ-dependent glucose uptake in myotubes. Lipids, 51 (8) 897–903 (2016)

 

･Kume, A., Kawase, K., Komenoi, S., Usuki, T., Takeshita, E., Sakai, H. and Sakane, F. The pleckstrin homology domain of diacylglycerol kinase η strongly and selectively binds to phosphatidylinositol 4,5-bisphosphate. J. Biol. Chem. 291, 8150–8161 (2016)

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･Liu, K., Kunii, N., Sakuma, M., Yamaki, A., Mizuno, S., Sato, M., Sakai,H., Kado, S., Kumagai, K., Kojima, H., Okabe, T., Nagano, T., Shirai, Y.and Sakane, F. A novel diacylglycerol kinase α-selective inhibitor,CU-3,induces cancer cell apoptosis and enhances immune response. J. Lipid Res., 57 (3), 368–379 (2016)

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･Sato, Y., Murakami, C., Yamaki, A., Mizuno, S., Sakai, H.and  Sakane, F.Distinct 1- and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes. Biochim. Biophys. Acta - Proteins Proteomics, 1864 (9), 1170–1176 (2016)

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総説

・Sakane, F., Mizuno, S. and Komenoi, S. Diacylglycerol kinases as emerging potential drug targets for a variety of diseases: An update. Research Topic in 2016 "Diacylglycerol Kinase Signalling", Front. Cell Dev. Biol. 4, Article 82, 1–8 (2016)

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2015年

原著

• Usuki, T., Sakai, H., Shionoya, T., Sato, N. and Sakane, F. Expression and localization of type II diacylglycerol kinase isozymes δ and η in developing mouse brain. J. Histochem. Cytochem., 63 (1), 57 – 68 (2015)

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• Shionoya, T., Usuki, T., Komenoi, S., Isozaki, T., Sakai, H. and Sakane, F. Distinct expression and localization of type II diacylglycerol kinase isozymes δ, η and κ in mouse reproductive organs. BMC Dev. Biol., 15 (1), 6 (article number, 10 pages) (2015)

 

• Komenoi, S., Takemura, F., Sakai, H. and Sakane, F. Diacylglycerol kinases η1 is a high affinity isozyme for diacylglycerol. FEBS Lett., 589 (11), 1272–1277 (2015)

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著書

•　坂根郁夫，第３節 ジアシルグリセロールキナーゼ，尾池雄一，佐々木雄彦編，Series モデル動物利用マニュアル　疾患モデルの作成と利用―脂質代謝異常と関連疾患　第６章　細胞内脂質シグナル関連因子，pp293－302，エル・アイ・シー，東京（2015）

 

 

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2014年

　原著

• Takeshita, E., Kume, A., Maeda, Y., Sakai, H. and Sakane, F. Diacylglycerol kinase γ is a novel anionic phospholipid binding protein with a selective binding preference.Biochem. Biophys. Res. Commun., 444 (4), 617-621 (2014)

 

• Kano, T., Kouzuki, T., Mizuno, S., Ueda, S., Yamanoue, M., Sakane, F., Saito, N., and Shirai, Y. Both C1 domain and a basic amino acid cluster at the C-terminus are important for the neurite and branch induction ability of DGKβ. Biochem. Biophys. Res. Commun., 447 (1), 89-94 (2014)

 

• Yamamoto, T., Sakai, H., Sakane, F. EF-hand motifs of diacylglycerol kinase α interact intra-molecularly with its C1 domains. FEBS Open Bio., 4, 387-392 (2014)

 

• Sakiyama, S., Usuki, T., Sakai, H. and Sakane F. Regulation of diacylglycerol kinase δ expression in C2C12 skeletal muscle cells by free fatty acids. Lipids, 49 (7), 633-40 (2014)

 

• Sakai, H., Kado, S., Taketomi, A. and Sakane, F. Diacylglycerol kinase δ phosphorylates phosphatidylcholine-specific phospholipase C-dependent, palmitic acid-containing diacylglycerol species in response to high glucose levels. J. Biol. Chem. 289 (38), 26607-26617 (2014)

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総説

•　坂根郁夫，ジアシルグリセロールキナーゼ　―DGKアイソザイムのさまざまな生理機能と病態との関連―，新井洋由編，生命を支える脂質―最新の研究と臨床，医学のあゆみ，248 (13), 1059-1063 (2014)

 

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2013年

　原著

• Hozumi, Y., Matsui, H., Sakane, F., Watanabe, M., and Goto, K. Distinct expression and localization of  diacylglycerol kinase isozymes in rat retina. J. Histochem. Cytochem. 61 (6), 462–476 (2013)

 

• Matsutomo, D., Isozaki, T., Sakai, H. and Sakane, F. Osmotic shock-dependent redistribution of diacylglycerol kinase η1 to non-ionic detergent-resistant membrane via the pleckstrin homology and C1 domains. J. Biochem., 153(2),179-190 (2013)

 

• Tanino, F., Maeda, Y., Sakai, H. and Sakane, F. Induction of filopodia-like protrusions by diacylglycerol kinase γ independent of its enzymatic activity in N1E-115 neuroblastoma cells: potential novel function of the catalytic domain of diacylglycerol kinase γ. Mol. Cell. Biochem.,373 (1),85-93 (2013)

 

• Kaneko, Y. K., Kobayashi, Y., Motoki, K., Nakata, K., Miyagawa, S., Yamamoto, M., Hayashi, D., Shirai, Y., Sakane, F. and Ishikawa, T. Depression of type I diacylglycerol kinases in pancreatic β-cells from male mice results in impaired insulin secretion. Endocrinology, 154 (11), 4089–4098 (2013)

 

• Sato, M., Liu, K., Sasaki, S., Kunii, N., Sakai, H., Mizuno, H., Saga, H. and Sakane, F. Evaluations of the selectivities of the diacylglycerol kinase inhibitors R59022 and R59949 among diacylglycerol kinase isozymes using a new non-radioactive assay method. Pharmacology, 92 (1–2), 99–107 (2013)

 

 

総説

• 坂根郁夫，最近明らかになってきたジアシルグリセロールキナーゼアイソザイムの制御する生理機能・病態形成と基質の多様性，日本応用酵素協会誌，48 ,11–18 (2013)

 

特許

・　  坂根郁夫，柳澤健二，加納英雄，神保孝一

　　　発明の名称：DGKα阻害剤を含有する抗癌剤

　　　特許権利者：国立大学法人 千葉大学

　　　特許出願番号（国内）：特願2008-508605

　　　出願年月日：2007年3月29日

　　　登録年月日：2013年11月29日

　　　登録番号：特許第5422204号

 

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2012年

　原著

• Mizuno, S., Sakai, H., Saito, M., Kado, S. and Sakane, F. Diacylglycerol kinase-dependent formation of phosphatidic acid molecular species during interleukin-2 activation in CTLL-2 T-lymphocytes: Development of a liquid chromatography mass spectrometry method for improving phosphatidic acid species detection. FEBS Open Bio, 2, 267–272 (2012)

 

• Takeuchi, M., Sakiyama, S., Usuki, T., Sakai, H. and Sakane, F. Diacylglycerol kinase δ1 transiently translocates to the plasma membrane in response to high glucose stimulation. Biochim. Biophys. Acta - Mol. Cell Res., 1823 (12),2210–2216 (2012)

 

• Sakai, H., Harun, F. M., Yamamoto, N., and Yuki, N. Contamination with gangliosides in brain-derived rabies vaccine may trigger Guillain-Barré syndrome. J. Neurol. Neurosurg. Psychiatry. 83 (4), 467-469 (2012)

 

• Takahashi, M., Yamamoto, T., Sakai, H. and Sakane, F. Calcium negatively regulates an intramolecular interaction between the N-terminal recoverin homology and EF-hand motif domains and the C-terminal C1 and catalytic domains of diacylglycerol kinase α. Biochem. Biophys. Res. Commun., 423 (3), 571–576 (2012)

 

• Takeishi, K., Taketomi, A., Shirabe, K., Toshima, T., Sakane, F. and Maehara, Y. Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras-Raf-MEK-ERK pathway. J. Hepatol. 57 (1), 77–83 (2012)

 

• Matsubara, T., Ikeda, M., Kiso, Y., Sakuma, M., Yoshino, K., Sakane, F., Merida, I. Saito, N. and Shirai, Y. c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr218. J. Biol. Chem. 287 (8), 5507–5517 (2012)

 

総説・著書

　・坂根郁夫．ジアシルグリセロール，トリアシルグリセロール．田中啓二，石浦章一，水島昇，谷口直之，遠藤玉夫，竹縄忠臣，伊藤俊樹，花岡文雄，塩見晴彦編．生化学事典，朝倉書店，東京，印刷中

 

• Sakai, H. and Sakane, F. Recent progress on type II diacylglycerol kinases: the physiological functions of diacylglycerol kinase δ, η and κ and their involvements in diseases. J. Biochem. 52(5),397-406 (2012)

 

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2011年

　総説・著書

• 坂根郁夫．甲状腺ホルモン（ヨウ素化チロシン誘導体）の生合成と生理作用機構．横山正孝監修．ヨウ素の化学と最新応用技術　第２編 生物環境とヨウ素　第７章，pp60－66，シーエムシー出版，東京（2011）

 

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2010年

　原著

• Knight, M. J., Joubert, M. K., Plotkowski, M. L., Kropat, J., Gingery, M., Sakane, F., Merchant, S. S., Modlin, R. L. and Bowie, J. U. Zinc binding drives sheet formation by the SAM domain of diacylglycerol kinase δ. Biochemistry 49 (44), 9667–9676 (2010)

 

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2009年

　原著

• Kai, M., Yasuda, S., Imai, S., Toyota, M., Kanoh, H. and Sakane, F. Diacylglycerol kinase α enhances protein kinase Cζ-dependent phosphorylation at Ser311 of p65/RelA subunit of nuclear factor-κB. FEBS Lett. 583 (19), 3265c3268 (2009)

 

･ Yasuda, S., Kai, M., Imai, S., Takeishi, K., Taketomi, A., Toyota, M., Kanoh, H. and Sakane, F. Diacylglycerol kinase η augments C-Raf activity and B-Raf/C-Raf heterodimerization. J Biol Chem. 284 (43), 29559-29570 (2009)

 

• Imai, S., Yasuda, S., Kai, M., Kanoh, H. and Sakane, F. Diacylglycerol kinase δ associates with receptor for activated C kinase 1, RACK1. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids, 1791 (4), 246–253 (2009)

 

　総説・著書

• 坂根郁夫，甲斐正広，安田智．最前線：最近明らかになってきたジアシルグリセロールキナーゼが関与する生理機能と病態．ファルマシア　45 (8), 780–784 (2009)

 

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​2008年以前の業績はこちら